ABSTRACT
Introducción: el estrés crónico afecta el equilibrio inmunológico alterando los niveles séricos de interleuquina-6 (IL-6) e interferón gama (INF-γ), dicha alteración afecta al sistema nervioso y al comportamiento humano. La masticación adecuada disminuiría dichos efectos. El objetivo del estudio fue determinar el efecto del estrés crónico y de la masticación sobre los niveles séricos de IL-6 e INF-γ. Métodos: experimento donde se emplearon 64 ratones Balb/c de 8 semanas de edad. Se dividieron en 4 tratamientos: Grupo NE: Masticación normal + estrés, Grupo N: masticación normal sin estrés, Grupo DE: Masticación deficiente + estrés, Grupo D: masticación deficiente sin estrés. Mediante test de ELISA se midió IL-6 e IFN-γ alfinal de la 4ta y de la 8va semana de tratamiento. Resultados: tanto la IL-6 como el IFN-γ fueron mayores en el grupo DE (p<0,05) al final de la 4ta semana. Al evaluarlos al término de la 8va semana se observó que en el grupo NE se incrementó la IL-6 respecto al resto de grupos (p<0,0001), y en el grupo DE fue donde se encontró mayor cantidad de IFN-γ (p<0,0001). Conclusión: el estrés crónico y la masticación deficiente incrementan los niveles séricos de IL-6 e IFN-γ. En cambio, la adecuada masticación disminuye el nivel de tales citoquinas al final de la cuarta semana de tratamiento.
Introduction: chronic stress affects the immune balance by altering the serum levels of interleukin-6 (IL-6) and gamma interferon (INF-γ), this alteration affects the nervous system and human behavior. Appropriate chewing would lessen these effects. The aim of this study was to determine the effect of chewing and chronic stress over serum levels of IL-6 and INF-γ. Methods: experiment in which 64 Balb/C mice of 8 weeks of age were used, they were divided into 4 treatments: Group NE: Normal chewing + stress, Group N: normal chewing without stress, Group DE: Chewing poor + stress, Group D: poor chewing without stress. IL-6 and IFN-γ were measured by ELISA after 4 and 8 weeks of treatment. Results: both IL-6 and IFN-γ were higher in the DE group (p < 0,05) at the end of fourth week of treatment. When evaluating the animals at the end of the eighth week of treatment, it was observed that in the NE group, the IL-6 was increased with respect to the rest (p < 0,0001) and the DE group showed more IFN-γ (p < 0,0001). Conclusion: stress and poor chewing increase serum IL-6 and IFN-γ. In contrast, appropriate chewing decreases the effects of stress on the increase of such cytokines at the end of the fourth week of treatment in animals.
Subject(s)
Animals , Male , Mice , Stress, Psychological , Interleukin-6/analysis , Interferon-gamma/analysis , Mastication , Enzyme-Linked Immunosorbent Assay , Chronic Disease , Mice, Inbred BALB CABSTRACT
There is urgent need to address illness problems caused by Salmonella enteric serotype Typhibacteria. The bacteria are deposited in water or food by human carrier and are then spread to other people in the area. In this research, a blood specimens were collected from typhoid fever patients, and serum levels of IFN-γ and IL-6 during the chronic and acute phase in typhoid patients group was determined according protocol kit and calculation, results were higher levels in chronic phase (137.187 ± 0.703.427 ± 206.545pg/ml respectively) and in acute phase were 128.787 ± 2.522, 137.733 ± 23.424 pg/ml, respectively with highly significant (P ≤ 0.01) than those in healthy control group. Salmonella infects hosts as diversified as human, animal, and plant
Subject(s)
Humans , Child , Adolescent , Adult , Middle Aged , Salmonella , Serology , Typhoid Fever/therapy , Blood Specimen CollectionABSTRACT
As leishmanioses compreendem um complexo de doenças causadas por parasitos intracelulares obrigatórios pertencentes ao gênero Leishmania. Consideradas como importante problema de saúde pública, sendo os cães domésticos os principais responsáveis pela manutenção da cadeia epidemiológica da doença, estima-se que mais da metade dos cães infectados não manifestam sinais clínicos da enfermidade. Avaliou-se o perfil de IL-10 e INF- γ de cães naturalmente infectados com Leishmania (Leishmania) chagasi no município de São Luís-MA. Foram coletadas 50 amostras, sendo 20 de animais positivos e sintomáticos para Leishmaniose Visceral Canina (LVC), 20 de animais positivos e assintomáticos e 10 de animais sabidamente negativos para a LVC. As amostras foram analisadas pelo teste imunocromatográfico rápido Dual Path Platform (DPP/Biomanguinhos®) e pelo ELISA (EIE/Biomanguinhos®) indireto para detecção de anticorpos anti-Leishmania. Após as confirmações dos testes, foi realizado o ELISA de captura para quantificação das citocinas IL-10 e INF-γ através do kit Milliplex MAP. Houve diferença estatística entre os grupos, observando um aumento de IL-10 em soros de cães sintomáticos para LVC, comparado com o grupo de animais assintomáticos, sugerindo que animais com essa expressão de IL-10 podem estar associados à susceptibilidade a doença. Assim como o aumento dos níveis de INF-γ observados em cães assintomáticos, comparado com o grupo de cães sintomáticos, poderiam estar relacionados à cronicidade da doença.(AU)
Leishmaniasis comprise a complex of diseases caused by intracellular mandatory parasites belonging to the genus Leishmania. Considered as an important public health problem, and domestic dogs are primarily responsible for maintaining the epidemiological chain of the disease, it is estimated that more than the half of the dogs infected do not show clinical signs of the disease. The profile of IL-10 and IFN-γ dogs naturally infected with Leishmania (Leishmania) chagasi in São Luís/MA was evaluated. Blood samples were collected from 50 animals, 20 from positive and symptomatic dogs for leishmaniasis canine (CVL), 20 from positive asymptomatic animals and 10 negative. Samples were analyzed by immunochromatographic test Dual Path Platform (DPP/Biomanguinhos®) and by indirect ELISA (EIE/Biomanguinhos®) for detection of anti-Leishmania antibodies. After the confirmation of the tests, the capture ELISA was performed for quantification of IL-10 and IFN-γ cytokines through the Milliplex MAP kit. There was a statistical difference between the groups, observing an increase of IL-10 in blood of symptomatic dogs for CVL, compared to the group of asymptomatic animals, suggesting that animals with this expression of IL-10 may be associated with susceptibility to disease. As well as the increase in IFN-γ levels in asymptomatic dogs, compared to the symptomatic dog group, could be related to chronicity of the disease.(AU)